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Part:BBa_I750003:Design

Designed by: Phillip Dodson   Group: iGEM07_Melbourne   (2007-06-16)


a short description of this part fcs


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
    Illegal NotI site found at 1727
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 1107
    Illegal NgoMIV site found at 1475
    Illegal NgoMIV site found at 1629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 667


Design Notes

design considerations


Source

ATGCGCTCGCTGAAGCCGCTTGCGGCCTTGCCCGATCGCTTGCTCGATCGCCTGGTGCACTGGGCGCGCG TGCGCCCCGAGCAGACCTTCATTGCCGCCCGCGAGGCCGGTGGCGACTGGCGCCTTGTCAGCTATGGGCA GATGCTCGACAGCGTGCGCGCCATCGCCCAGGGCCTGCTTCGTTACGGCCTGTCGGCCGACAAGCCGCTG GCGCTGCTCTCGGGCAATGACATCGAACACCTGCAACTGGCCCTGGGCGCGATGTACGCCGGGATTCCCT ATTGCCCGGTGTCGCCGGCCTATTCGCTGCTGTCCCAGGACTTTGCCAAGCTGCGCCATGTCTGCGACCT GTTGCAGCCGGGGCTGGTGTTCGTCAGCGACGCCAGCGCCTACCAGCGGGCCATCGACGCGGTGCTGCCG CCCGAGACGCCGCTGATCAGCGTGCGTGGCCAGGTGCCGGGGCGGCGCCAGGCGAGCTTCGCCAGCCTGC TGGCCGAGCCCGGTGGCGCCGAGGCCGATGCGGCCTTTGCCGCCACCGGCCCTGACAGCATCGCCAAGTT TCTCTTCACCTCGGGCTCGACCAAGCTGCCCAAGGCGGTGATCACCACCCAGCGCATGCTCTGCGCCAAC CAGCAGATGCTGCTGCAGACCTTTCCGGTGTTCGGTGAAGAGCCGCCGGTGCTGGTGGACTGGCTGCCGT GGAACCACACCTTCGGCGGCAGCCATAACGTCGGCATCGTGCTCTACAACGGCGGTACTTTTTACCTGGA CGAGGGCAAGCCCACGGCCCAAGGCTTCGCTGAAACCCTGCGCAACCTCAAGGAGATTTCCCCCACCGCC TACCTGACCGTGCCCAAGGGCTGGGAGGAACTGGTCAACGCCCTGGAGCAGGACGCCGAGCTGCGCGAGT GTTTTTTCAAGCGCATGAGCCTGTTTTTCTTCGCCGCCGCCGGGCTTTCGCAAAGTGTCTGGGATCGCCT GGACCGGGTCGCCGAGCAGCATTGCGGCGAACGCATCCGCATGATGGCCGGGCTCGGCATGACCGAGGCC GCGCCGTCTTGCACCTTCACCACCGGACCGCTGTCGATGGCCGGTTACATCGGCCTGCCGGCGCCGGGTT GCGAAGTGCGCCTGGTGCCGGTGGATGGCAAGCTCGAAGGGCGTTTTCGCGGGCCGCACATCATGCCCGG CTACTGGCGCGCGGCGCAGCAGACCGCCGAGGTGTTCGACGCCCAGGGCTTCTACTGCTCGGGGGATGCG CTCAAGCTCGCCGACGCCGCCGACCCGCAACTGGGGCTGATGTTCGACGGGCGCATCGCCGAGGACTTCA AGTTGTCGTCCGGGGTGTTCGTCAGCGTCGGACCGCTGCGCAATCGCGCGGTGCTCGAAGGCGCGCCCTA TGTCCAGGACCTGGTGGTGGCCGCCCCCGACCGCGAGTGCCTGGGGGCCCTGGTGTTCCCGCGCCTGTAT GAGTGCCGGCGGCTGGCGGGGCTGGGCGCCGAGGCCAGCGAGGCCCAGGTGCTGGCCAGCGCGCCGGTGC GCCAGTGGTTCGGCGACTGGTTGCAGTGCCTGAATCGTGAGGCCAGCGGCAATGCCAGCCGCCTGGAGTG GATCGCGCTGCAGGTGGAGCCGGCGTCCATCGACCGTGGGGAGATCACCGACAAGGGCTCGATCAATCAG CGCGCGGTGCTGCAGTGGCGCGCCGAGCAGGTCGAGAACCTGTATCGCGGCCGCGAGCCGTCGATCCTGC GCGCCGAGCCCAAGCCATGA


References